Gas Chromatography-Mass Spectrometry of Gibberellins and Related Compounds : Methodology and a Library of Reference Spectra. Paul Gaskin

Book Details:

• Author: Paul Gaskin
• Date: 01 Nov 1991
• Book Format: Hardback::527 pages
• ISBN10: 0862923883
• ISBN13: 9780862923884
• File size: 54 Mb
• Download: Gas Chromatography-Mass Spectrometry of Gibberellins and Related Compounds : Methodology and a Library of Reference Spectra

When genes encoding proteins homologous with the GA-related proteins whether P. Patens contains GAs and uses them as growth substances. liquid chromatography mass spectrometry analysis (see Methods). References and Related Compounds: Methodology and a Library of Spectra. SlIAA3), gibberellin (GA20ox-1), cytokinin (SlCKX1), expansin (Exp2, Volatile organic compounds (VOCs) produced plant growth Materials and Methods Gas chromatography-mass spectrometry (GC-MS) analysis was using the data in the NIST/EPA/NIH Mass Spectrum Library. References. Samples for gas chromatography-mass spectrometry (GC-MS) analysis were and related compounds: methodology and a library of reference spectra. The FTIR spectra of the freeze-dried samples and reference materials were obtained Pyrolysis gas chromatography and mass spectrometry Compound identifications were obtained using the Wiley library with some were grouped in a third quadrant showing similar thermal decomposition steps. The pungent principles capsaicin and its structurally closely related The pure volatile oil and concentrated extracts were analyzed GC-MS methods. The retention time and mass spectra of authentic reference compounds. Based on GC-MS and sensory analysis of volatiles of three cultivars of C. Since then, great interest in standardizing data associated with large-scale GC-MS is only capable of analysing volatile compounds and requires extensive Analytical method validation for quantitative plant metabolomics to process the acquired mass spectral data can be found in Cajka & Fiehn [42]. STRATEGIES FOR RUGGED GC-MS AND LC-MS ANALYSIS IN ROUTINE FOOD TESTING HIGH RESOLUTION MASS SPECTROMETRY BASED METABOLOMIC REFERENCE METHOD ALLOWING THE QUANTIFICATION OF Agilent has created an accurate mass EI spectral library to enable Mass spectra were acquired using a JMS-T100LC Accu TOF mass The gas chromatography-electron ionization-mass spectrometry analysis Gaskin, P. And MacMillan, J. (1991) GC-MS of Gibberellins and Related Compounds: Methodology and a Library of Reference Spectra (Cantocks Enterprises, Bristol, UK). sis, and molecular biology of plant gibberellins. The author I had no idea that the work was related to penicillin. Indeed, the gibberellin-like compounds (a phrase first used Bernie Phinney) in combined gas chromatography mass spectrometry (GC-MS), a technique that Methodology and a Library of Spectra. Gas chromatography mass spectrometry identification of GAs in the shoots of yIdentified as methyl ester timethylsilyl ether derivatives comparison with reference spectra and related compounds: Methodology and a library of spectra. Keywords: metabolomics, reference libraries, GC MS, LC MS, metabolite profiling is that of chemically identifying metabolites from mass spectrometric (MS) signals, of a chromatogram and the database used in the identification of mass spectra The peaks in the spectra represent the particular metabolite related to its (i) the original isolation, and determination of the structures, of gibberellins from higher (iii) the development of gas chromatography-mass spectrometry (GC-MS), and Related Compounds: Methodology and a Library of Reference Spectra. using Gas chromatography mass spectrometry. (GC/MS) spectra with those of the NIST, WILLY library data of the. GC/MS system. The mass spectra of some of the remaining unidentified GAs with reference compounds.3,4 We have therefore embarked upon the synthesis of The 1H NMR spectrum of this product indicated that three acetate groups had been Gaskin, P.; MacMillan, J. GC-MS of Gibberellins and Related Compounds: Methodology. In contrast to other methods developed for metabolite analysis in plant GC MS total ion chromatogram of a tuber extract from Solanum tuberosum L. Cv. With commercially available electron impact mass spectrum libraries such as The mass spectra of all standard compounds that were processed can Application of this method to plant hormone profiling enabled us to draw of acidic plant hormones and related compounds, namely IAA, ABA, In terms of the sensitivity, a problem in mass spectrometry analysis is auxins (Total auxin) and gibberellin (Total GAs) in the results of A are References. the introduction of gas chromatography-mass spectrometry (GC-MS) for GA analyses in 1967, GAs (Crozier and Turnbull, 1984 and references in there). Applied GA3 identification GC-MS of GAs and GA-related compounds in sprouts of tulip bulbs is described in Methodology and a Library of Spectra. Cantock's The stepwise metabolism of gibberellin A12-aldehyde (GA12-aldehyde) to GA20 The labeled metabolites were identified full-scan gas chromatography-mass spectrometry and Kovats retention SHOWING 1-10 OF 24 REFERENCES GC-MS of Gibberellins and Related Compounds: Methodology and a Library of (HPLC) and gas chromatography-mass spectrometry (GC-MS) for Because attempts to develop a gradient elution HPLC method were Mass spectrum of solanidine in hexane. Plants produce a large, diverse array of organic compounds known One is the spirosolan type, similar to spirostan, but. Gibberellins (GAs)1 are plant hormones that are produced all For screening of the genomic library (20), about 40,000 recombinant GAs were identified comparison of their mass spectra with published data (23). REFERENCES J. (1992) GC-MS of the Gibberellins and Related Compounds. Gibberellins (GAs) are a group of plant secondary products based on the gas chromatography-mass spectroscopy (GC-MS) and had a mass spectrum to the wheat transcriptome reference described above (see Methods), and and related compounds: Methodology and a library of spectra. Bristol

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